Western blotting is an essential analytical technique used in molecular and cell biology. A western blot is a laboratory method used to separate and detect specific proteins from a complex protein sample. Proteins are divided by gel electrophoresis based on the molecular weight of proteins, which form bands on a gel and are then transported onto a nitrocellulose or polyvinylidene fluoride membrane. The steps in western blotting are tissue preparation, gel electrophoresis, transfer of proteins onto the membrane, blocking to prevent membrane and antibody interactions, incubation of membrane, and antibody-based detection of proteins.
The global western blotting market size was growing at a significant CAGR during the forecast period, 2020-2027. The critical factors are the rising prevalence of HIV/AIDS and increasing R&D investments. Additionally, the increased efforts and funding in research and development by several pharmaceuticals and biotechnology companies will spur the global western blotting industry in the future periods. Furthermore, the rising burden of chronic & Lyme disease and rapidly expanding applications of western blotting in the field of proteomics is expected to witness remarkable growth of western blotting. The increasing production of genetically modified crops and growing range of industry-academic examination collaborations are expected to propel the demand for western blotting over the coming years.
Moreover, the growing genetic science market and biotechnology sector are positively impacting the market for western blotting. Besides, the increasing demand for personalized medicine and new product launches fuel the western blotting market. The speedily developing healthcare system and increased demand from the medical diagnostics sector will bolster the global western blotting market share growth.
The Western Blot is a reliable qualitative immunoassay in which antibodies particular to the B. burgdorferi antigens are visualized on a membrane strip. It is a qualitative test and is usually more specific and sensitive than the IFA and ELISA tests. Western blot delivers both confirmatory results in the field of research and diagnostic testing. As per the report by CDC, nearly half a million people are diagnosed with Lyme disease annually. Lyme disease is the most usually reported tick-borne illness in the United States. Infections predominantly occur in the north-central and northeast regions of the United States. According to research published in 2018 by Carnegie Mellon University, Lyme disease is assumed to spread in the United States due to climate changes. Several studies have revealed that western blot IgM has 94-96% specificity towards the patient with the clinical symptom of Lyme disease. Western blot is used in the confirmatory test for HIV followed by ELISA test. Western blot was the particularly gold standard for the diagnosis of HIV-1 infection. According to data published on HIV.gov in 2016, approximately 38,700 people were newly infected by HIV. Therefore, the rising prevalence of Lyme and HIV is likely to accelerate the market for western blotting during the forecast period.
Recently, the outbreak of COVID-19 has vigorously affected the healthcare system of developed and developing countries globally. The growing nervousness regarding the shortage of essential medical life-saving devices and other necessary medical supplies to block the spread of this virus and provide optimum care to the infected also widens. Additionally, the manufacturing process’s surge for a wide range of test kits, such as antibody tests, will enhance the demand for western blotting in the healthcare industry.
Based on the product, the global western blotting market is segregated into instruments and consumables. The instruments segment is segmented into gel electrophoresis instruments, blotting systems, and imagers. Further, the consumables segment is categorized into consumables, reagents, and kits. The consumables segment will witness the highest growth over the analysis timeframe due to the rising cell & molecular biology research activities and growing prevalence of target diseases requiring western blotting for research and diagnosis.
The instruments segment will grow significantly due to the growing availability of automated instruments for conducting western blot, which helps in facilitating the rapid diagnosis of chronic diseases. In addition, the ever-increasing adoption of electrophoresis blotting systems in research activities drives the market. Electrophoresis blotting efficiently identifies a specific protein in a given sample while employing an enzyme or fluorescence-labeled primary antibody to bind to its particular antigen. As a result, it has high demand in various applications.
The global western blotting market is classified into biomedical & biochemical research, disease diagnostics, agriculture, and other applications based on the application. The disease diagnostics segment is expected to be the highest revenue share for western blotting during the forecast period. The segment’s growth is primarily attributed to the increasing prevalence of HIV, Lyme disease, HSV, HBV (Hepatitis B Virus) infection, BSE (Bovine Spongiform Encephalopathy), mad cow’s disease, and advancement in the medical diagnostics sector. In addition, the western blotting technique serves as a diagnostic method for various infectious, autoimmune, and oncological diseases. In the infectious diseases area, protein detection through western blotting supports multiple conditions, like visceral leishmaniasis.
The biomedical & biochemical research segment will witness a lucrative growth rate in the estimated period due to the growing usage of R&D activities and government funding in research related to proteomics. In addition, the routine utilization in research & clinical settings for the prevention and treatment of diseases drives the market.
Based on the end-user, the global western blotting market is segmented into academic & research institutes, pharmaceutical & biotechnology companies, diagnostic centers, and other end users. The theoretical and research institutes segment will dominate the global western blotting market by 2027 due to the surge in R&D activities conducted by academic institutes and research centers.
The pharmaceuticals & biotechnology companies were assigned for the largest share of the western blotting market in 2019. It is due to the increasing use of western blotting in the clinical trial setting and drug discovery & development.
The global western blotting market is segmented into North America, Europe, Asia Pacific, South America, and Middle East & Africa by geography. North America is estimated to project considerable growth over 2020-2026. It is due to leading industry players, the increasing prevalence of HIV and Lyme disease, and the growing number of collaborative programs for biomedical research.
The Asia Pacific will capture a significant share of the western blotting market by 2027. Due to the increasing government interest in the biomedical & biotechnology industry, rising production of genetically modified crops, and growing pharmaceutical industry.
Thermo Fisher Scientific Inc., Bio-Rad Laboratories Inc., Merck KGaA, PerkinElmer Inc., Danaher Corporation, GE Healthcare, Advansta Inc., LI-COR Biosciences, Bio-Techne Corporation, F. Hoffmann-La Roche Ltd., and Cell Signaling Technology Inc. are the key players in the global western blotting market.
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